BIOL 205 Lecture Notes - Joshua Lederberg, Edward Tatum, Bacterial Conjugation
8 Nov 2013
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Document Summary
Everything you wanted to know about bacteria sex but were afraid to ask . Gene regulation and gene products: modern dna technologies are all derived from bacteria and their viruses. If competent: can take up dna from envmt (transformation) donating plasmid: extra chromosomal dna donating chromosomal dna virus infects w/ some of bacterial genome. Bacterial colonies, each derived from a single cell w/ nutrients. Distinguishing lac+ and lac- by using a red dye. How would you isolate a bacteria mutant that can"t grow on minus arginine media ? colonies that fail to grow on the minus arginine plates are potential arginine auxotrophs (mutant) Joshua lederberg and edward tatum (1946) discovered a sex-like process in what became the main model of bacterial genetics. Mixing bacterial genotypes produces rare recombinants: q. F plasmid: low copy number restriction also takes energy. And pilus sometimes used by viruses to grasp onto bacteria.
