BIOL 205 Lecture Notes - Har Gobind Khorana, Pyrococcus Furiosus, Dna Microarray
22 Nov 2013
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Need to achieve two things: (i) immobilize (array) thousands of dna probes specific for each individual mrna gene product (ii) label mrna populations. Robots designed to spot 44,000 individual dna spots. The probes can be cdnas (~1kb) or oligonucleotides (20-70 mers) Advantage to oligonucleotides: chemically synthesize and get exact nt sequence. Insufficient hybridization array of 1046 cdnas probed with fluorescently labelled cdna made from bone marrow mrna. Tells you whether you need new protein synthesis to change transcript level. Each row is a different gene, each column a different time point increase. Personalized medicine: diagnose changes, predict disease or infection. Predict drug efficacy or toxicity in individuals. Problems: if insurance companies for info that you have mrna for certain disease and deny insurance. Introducing double stranded rna inhibits the respective gene. Hard to knockout genes in mammalian cells: degrades rna, blocks translation, recruits chromatin remodelers to block further transcription. Dicer cleave into exactly 21 nucleutides silencing rnas picked up by
