BIOL 205 Lecture : Lectures29-30
22 Nov 2013
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Biol 205: week 10-11, lecture 29-lecture 30 (beginning of lecture 30) In 2003 the human genome project completed an initial draft sequence of the human genome after ~13 years and a cumulative cost of ~ million. 2003 sh. 10/base: costs dropped sharply over the course of project due to improvements in sequencing technology especially replacing gels with capillary electrophoresis. 600 bp of sequence possible in each run. Contig which then overlap for an entire sequence. Craig venter developed shotgun sequencing founded the institute of genomic research (tigr) founded celera genomics sequenced his dog! predicts that future will have everyone sequenced! Sanger institute: announcement 10,000 human genomes in next 3 years. But 1-fold helps computer look for overlaps. Gaps in human genome still not filled: ex repetitive sequences that are hard to clone. Less than 3% encodes exons of mrnas! 8, 619 based on computer prediction average orf size ~ 27 kbp. 42% of orfs have no known or predicted function!
