BMS 860 Lecture Notes - Lecture 8: Apaf1, Caspase 8, Cytosol
26 Feb 2017
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Document Summary
P53 is the most studied single gene in history. P53 is a tumour suppressor gene located on chromosome 17p13 that encodes p53 protein. P53 protein is a transcriptional factor that forms homotetrameric protein complex. Normal p53 protein is activated by a number of cellular stressors including dna damage and oncogene activation. Activation of p53 leads to cell cycle arrest, apoptosis and/or senescence of the affected cells. 50% of all tumours exhibit the loss of p53 function: mutations/deletions and loh. Inactivation by viral oncogenes (p53: expression of dominant negative mutant p53 proteins, overexpression of p53 regulating protein mdm2. Sv40 is animal virus that causes cancer, large t antigen involved in transformation. Dr. lane was developing antibodies to detect large t antigen in sv40 transformed cells nih3t3. Immunoprecipitation the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein.
