BMS 150 Lecture Notes - Lecture 9: Frederick Sanger, Sanger Sequencing, Polyacrylamide Gel Electrophoresis
Document Summary
Set of dna sequences carried by individual genome is contained in each normal somatic cell and contains two copies of each gene. Bioinformatics uses genomics to analyze nd genomic information. Proteomics is the study of functions and interactions proteins. A genome is an organisms complete set of deoxyribonucleic acid. We all have one, all of the dna all of the hereditary material of an organism. Any dna sequence that we can determine can be compared to databases. To create maps of human and other genomes. To determine the locations of genes on chromosomes. Compile lists of expressed and unexpressed genes. To identify proteins encoded by genes and their functions. To compare genes and their proteins among species. To set up and manage data bases of genomes. Need to sequence both directions to make sure that you are right. Dna target needs to be sub cloned into a plasma or virus vector.