BIOL 2321 Lecture Notes - Lecture 24: Spheroplast, Real-Time Polymerase Chain Reaction, Deoxycytidine Triphosphate
10 Jan 2016
School
Department
Course
Professor
Document Summary
Study online at quizlet. com/_1qc898: how does transcription terminate in. Rna pol i: how does transcription terminate in. Termination depends on polymerase-specific termination factors that recognize and bind specific dna sequences at the end of the rrna transcription unit. These sequences are often repetitive , e. g. , mammals have a termination sequence that is 18-bp long, repeated 10 times. shotgun sequencing a genome: initiation of transcription in bacteria, transcription termination in. Random primers dna labeling system: denature dna fragments. 2 anneal random sequence oligonucleotides to both strands: extend klenow fragment by polymerase, using three cold nucleotides and one radioactively labeled nucleotide provided, denature to ssdna. Randomly sheared dna used to make a genomic library. Clones picked at random from the library and sequenced using universal primers. Individual sequence reads assembled in computer to produce contigs. Transcription begins with the binding of rna polymerase to the promoter in dna.
