BISC 101 Lecture Notes - Lecture 5: Alpha-Amylase, Methylene Blue, Test Tube
15 Dec 2017
School
Department
Course
Professor
Document Summary
Increasing the temperature will increase the rate of enzyme-catalyzed reactions. Design an experiment to test the temperature effect on the enzyme alpha amylase. Alpha amylase is an enzyme that breaks down starch. By adding starch, alpha amylase, and iodine, we can test how long it takes to break down all the starch. When all the starch has been broken down, the iodine indicator will be yellow, signifying that there is no starch present. Vary the temperatures to see how it affects the rate at which the enzyme works. *reaction rate = 1/(time taken for reaction to reach completion) Low temperature results in molecules moving slower, so the enzymes encounter the starch less, thus slowing down the rate. The reaction rate seemed to increase exponentially as the temperature increased from 0 to. The enzyme is likely found in a human/mammalian body, and the body has an internal temperature of 37 c, therefore the enzyme will function most optimally at that temperature.
