BIOCH200 Lecture Notes - Lecture 7: Gc-Content, Cell Nucleus, Base Pair

• School: University of Alberta
• Department: Biochemistry
• Course: BIOCH200
• Professor: Jonathan Parrish

Document Summary

Biochem 200 - lecture #7 - nucleic acids pt. Melting curves and base composition: g-c pairs have more base-stacking interactions. Increase stability of the structure: more absorbance = increased kinetic effect. Relationship between tm and the gc content of dna: high gc content = elevated tm. Regions of dna rich in at base pairs denature most readily: at base pairs easier to separate. Ph and salt concentration: changing ph affects tm. Due to effect on protonation state of dna. Ions shield negative charges on phosphate backbone: cations decrease repulsion through electrostatic attraction. Helps to have positively charged proteins (eg. histones) The denaturation and renaturation behaviour of nucleic acids enables hybridization: hybrid duplex is combining 2 samples of dna together, tm also depends on complementary strands (how close the primary structures are) and. This is because na+ is positively charged and so promotes the formation of the ph/[salt] Ph = ss = temperatures dsdna.