BIOCH200 Lecture Notes - Lecture 14: Cooperative Binding, Peptide, Coordinate Covalent Bond

• School: University of Alberta
• Department: Biochemistry
• Course: BIOCH200
• Professor: Jonathan Parrish

Document Summary

Biochem 200 - lecture #14 - protein function pt. Involved with coordination bond to heme and prevents oxidation. Binds to oxygen and is involved in: hise7 specificity of oxygen binding. Oxygen (o2) binding to the heme group in myoglobin: occurs at 6th coordinate position of the fe 2+ ion, distal histidine (his e7) O2 specificity (more molecules (eg. c o) favourable for oxygen) Does not interact directly with iron or heme: proximal histidine (his f8) **affinity: how well something binds to another molecule. If a affinity and b affinity, specificity (a)! The oxygen binding site in myoglobin: a good binding ligand will ultimately encourage oxygen binding, binding sites: optimize binding specificity and affinity. Specificity and affinity iclicker: distal histidine decrease co binding by physically blocking binding. Oxygen binding to myoglobin: when o2 is consumed, it releases easily, at rest, o2 bound to myoglobin and myoglobin binds efficiently to oxygen, kd 2. 5 torr. > kd = less oxygen being bound.