BIOCH200 Lecture Notes - Lecture 1: Integral Membrane Protein, Glycolysis, Fumaric Acid
10 Dec 2017
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Document Summary
The citric acid cycle occurs in the mitochondrial matrix. The citric acid cycle: condensation of acetyl coa with oxaloacetate to form citrate in the presence if citrate synthase. It is an irreversible reaction as g << 0. Isocitrate is converted to a-ketoglutarate in the presence if isocitrate dehydrogenase. It is also an energy capture stage as nadh is produced. It is the first oxidative carboxylation stage and co2 is given off: a-ketoglutarate is converted to succinyl coa in the presence of a-ketoglutarate dehydrogenase. It is the second oxidative decarboxylation and co2 is given out. Nadh is produced hence it is an energy capturing stage. Coa is a high energy intermediate as it has a thioester bond: substrate level phosphorylation. Energy capturing reaction: succinate is converted to fumarate in the presence of succinate dehydrogenase. Takes place in the integral membrane protein (complex ii): hydration, malate is converted to oxaloacetate.
