BIOL201 Lecture Notes - Lecture 16: Gtpase, Cyclic Adenosine Monophosphate, Antibody
Document Summary
Expression plasmid: manipulated plasmids to insert new pieces of dna e. g a gene, and then put the plasmid into cells (of all types) to express that gene. Transfect: the process of getting the plasmid into a eukaryotic cell (transformation in bacteria) Insulin receptor is autophosphorylated (tyrosine kinase associated receptor) Irs-1 is recruited to the receptor and is phosphorylated: irs-1 uses. Sh2 domain = structured part of a protein that always binds to phospho tyrosine. Tyrosine kinase domains phosphorylate tyrosine amino acids irs-1 (insulin receptor substrate- anything a receptor can bind to). Creates more signals, more amino acids, as long as the molecules have the. Grb 2 is an adaptor protein with an sh2 domain (hangs on to receptor and creates a hanging spot for sos- it is just a scaffold). Sos is an adaptor protein that binds to grb2 and acts as a guanine exchange factor (associates with a monomeric g protein and kicks out a gdp molecule,