BIOL207 Lecture Notes - Lecture 30: Metagenomics, Rna-Seq, Sanger Sequencing
21 Apr 2018
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Document Summary
Limitations of automated sanger sequencing: requires many copies of the template dna. A genome contains few copies of a specific target. Dna had to be isolated, broken down and incorporated into bac vectors. Very expensive: each reaction can only generate 700 nucleotides worth of data. Use a kit to break it into small fragments. Load the fragments into the machine and turn it on. Once inside dna fragments are isolated from each other. Sequence assemble is done by a software. Basically, takes the little sequences and joins them together: comparison between dna sequencing methods. Cheap if small sequence needs to be sequenced. Expensive: using next generation dna sequencing to sequence humans. A persons dna can give information about their susceptibility to different diseases and response to various treatments. Treatments can now be designed around the gentic defects that lead to the cancer: using next generation sequencing to sequence other organisms: De novo sequencing: organism sequenced for the first time.
