BIOL207 Lecture Notes - Lecture 34: 23Andme, Fluorescent Tag, Oligonucleotide
21 Apr 2018
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Human genetic testing: single nucleotide polymorphisms (snps) There is a change in one single base pair. Original allele: ancestral allele and minor allele: new allele. Do not affect genes that are near or within. Research groups choose people from different ethnic backgrounds to sequence. Different populations have mutations, and many have the minor allele. Snp entered into snp data(cid:271)ase as (cid:858)referen(cid:272)e snp(cid:859: microarray technology: Technology used to quantify dna or rna molecules. A piece of glass or silicone with short pieces of dna stuck to its surface. Rna molecules just need to be attached to fluorescent molecules. Pour the labelled sample into the microarray. Microarray is put into a microarray reader to take a digital photo of the fluorescent. If the piece of labelled nucleic acid is complementary to a spot, it will hybridise ssdna is covalently attached to fluorescent dye spots. Presence of fluorescence: the presence of complementary sequence in the sample.
