GENET301 Lecture Notes - Lecture 9: Population Bottleneck, Heteroplasmy, Restriction Digest
23 Sep 2016
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Document Summary
In yeast, both parents contribute to the cytoplasm of the zygote mtdna inheritance is biparental. However, in many organisms, the female contributes (by far) most of the cytoplasm to the zygote. Shown in some species that mitos/mtdna from the male is actively degraded in fertilized eggs. But, it has also been shown in a variety of species that, rarely, paternal mtdna can be detected in offspring. Various studies of animal mtdna reveal sequence differences in mtdnas of different individuals of a population. These questions have been addressed in varying degrees in studies using various organisms. One of the first done was on holstein cows. Although rare, a mtdna rflp was found in certain animals of a herd (assume a silent/neutral mutation - no effect on mt function) In some animals, mtdnas contained an extra hpaii restriction enzyme site. Gives two shorter bands in the restriction digest (s type mtdna - shorter fragments)
