NEURO410 Lecture Notes - Lecture 7: Mass Spectrometry, Isoelectric Point, Proteome
12 Oct 2016
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Functional genomics: 2003 human genome sequencing finished. Post-omic era to understand how they actually work. Metabolomics: lipids molecules are analyzed vs. hydrophilic metabolites: amount of data from these disciplines can be stored, analyzed and compared (via math models to merge info) (cid:894)cartoo(cid:374)(cid:895): (cid:862)a(cid:374)d that"s why we (cid:374)eed a co(cid:373)puter(cid:863) Amount of data = huge; unbearable to manually analyze. Cartoon showing traditional biochemistry (fishing pole) and proteomics (large net) Ab individually; proteomics allow analysis of 10,000s proteins at once. Clinical samples ie csf of normal vs disease. May lead to biomarkers (variable with presence of disease); can specifically look for that protein and determine progression. Proteomics tech = very powerful but complex with many steps. + controls) prep step (extract all proteins from sample, leading to complex sample to analyze b/c all proteins are there, mixed) protein separation (simplify ie divide depending on biochemical or biophysical properties, including liquid.
