CMMB 413 Lecture Notes - Lecture 4: Cytogenetics, Allosome, Y Chromosome
23 Feb 2019
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The principles of clinical cytogenetics part 3. Genomic hybridization methods involve the use of probes that are complementary to target sequences or chemically linked to fluorescence dye or between ratios of a patient sample as well as reference sample (cgh and microarrays) X axis: indicates different types of chromosomes, sometimes will only focus on one chromosome. Y axis: tells the ratios between the patients and the reference sample. Each window width indicates how many genes are protein-coding. Chromosome 18: when ratio is 1 or 0. Know there is a duplication, by looking at the blue area, know that almost entire chromosome 18 is duplicated because the whole width is blue. Height of blue colour indicates the amount of duplication or deletion. 1. 5 on y axis, means that the entire chromosome is about 1. 5x duplicated. Using the opposite gender of the sample.
