CMMB 421 Lecture Notes - Lecture 8: Bacteriophage Ms2, Microviridae, Rna-Dependent Rna Polymerase
28 Dec 2019
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Document Summary
Members of the leviviridae have small icosahedral capsids (t=3) and short single molecules of ssrna(+) as their genome. They encode 4-5 proteins, only two of which are present in capsids. Genomes are immediately translated to give coat protein, then replicase and lysis protein. Replicase replicates genome, but requires other (host) proteins to do this. Maturation protein is translated from new + rna molecules. All of these processes are regulated by rna folding and secondary structure so that timing of synthesis and abundance of proteins are optimized. Most studied example is e. coli phage phix174. T = 1, therefore only have pentamers. Have more different proteins that make up the capsid because the monomers that make up protomers are different proteins. H protein: internal protein located under each point of icosahedral. Because there are more proteins in the actual capsid, there are also more genes. Overlapping genes in this plasmid similar to lysis protein being hidden in orf of ms2 phage.
