BIOL 461 Lecture Notes - Lecture 4: Mutation, Reverse Genetics, Genetic Screen
12 Feb 2018
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Biol 461 lecture 4 heidelberg screen. Forward: do a search for mutants in a process that you want to study, but you have no idea what the genes are. Look for mutants that are affected and produce a phe(cid:374)ot(cid:455)pe that"s of interest: no idea what the genes are, biased just for the phe(cid:374)ot(cid:455)pe that (cid:455)ou"(cid:396)e looki(cid:374)g fo(cid:396) Reverse: still trying to infer the function of the gene product based on a phenotype (logic/analysis is the same) Forward and reverse genetic approaches in drosophila. (a) forward genetics uncovers the genetic basis of phenotype. Mutagenesis by any means (e. g. x-rays, chemicals or transposons; indicated by a lightning bolt) is used to generate mutant flies with aberrant phenotypes (indicated by the red fly), which are used as a starting point for gene discovery. T-dna insertion: identify the gene, map based cloning, clone identification from transposon tag or.
