KNES 259 Lecture Notes - Lecture 3: Spindle Apparatus, Low-Density Lipoprotein, Microorganism

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Passive transport doesn"t require any energy and it involves either simple or facilitated diffusion. Always uses energy, usually from atp to carry out transport. Primary: directly uses energy from hydrolysis of atp. Secondary: uses electrochemical gradient set up by primary active transport to drive transport of second substance. Always requires a carrier, therefore at a point when all carriers are working, there will a maximum reached, at which rate cannot be increased anymore. Firstly there is binding of molecule to carrier, then phosphorylation of atp into adp (energy stored in phosphate group). When carrier is loaded, it releases phosphate, and so it changes conformation, so that molecule can be released against concentration gradient. 40% of atp produced per cell is used to keep this pump functioning. Pump never stops because we always have these ions leaking through membrane down their electrochemical gradient. Inside the cell, 3 na+ bind to pump, alongside there is atp -> adp (phosphorylation of.

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