ANSC 4050 Lecture Notes - Lecture 7: Commensalism, Fusion Protein, Escherichia Coli
18 Sep 2017
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Document Summary
Inefficient release into the supernatant: degredation of membrane and/or cell wall associated proteinase, degradation of secreted proteinases. Protein folding: 2 major types of chaperones, molecular chaperones, ex. Protein disulfide isomerase: an enzyme in the er of euaryotes, catalyzes the correct formation of disulfide bonds between cysteine residues in protein to help proper folding. Degradation of exported protein by cell associated or secreted proteases: many gram positive bacteria secrete proteases into the supernatant, thus recombant proteins can be degraded in the supernatant. Systems: food grade (safe, fast growing, easy to manipulate genetically, able to grow in cheap media, minimum production of secreted proteases. Increase sow productivity: problems with early weaning, underdeveloped digestive capacity in newly weaned pigs leads to, low feed intake, susceptible to intestinal infections, both lead to diarrhea and effect on growth, current practice solution. Ivolved abnormal t cell responses to commensal micro flora. Il-10 down regulates inflammatory cascades and is used clinically to treat.
