ANSC 4050 Lecture Notes - Lecture 2: Small Interfering Rna, Polyadenylation, Eukaryotic Initiation Factor 3

• School: University of Guelph
• Department: Animal Science
• Course: ANSC 4050
• Professor: Dr. Julang Li

Document Summary

Phi-x 174 (phage) : 5,386 bp, 10 genes bacterial: approx. 0. 4 billions, 37,544 genes: the human genes comprise only approx. Syn 3. 0 has the fewest genes of any known free-living organism. Two steps (levels) of gene expression: transcription, translation. Dna (replication) (transcription) mrna (translation) protein. 5" promoter exon intronexonintronexon 3" promoter : a region of dna involved in gene expression control. Like a switch that can turn gene expression on and off to(cid:449)ards the (cid:1009)" regio(cid:374) of the ge(cid:374)e. (cid:271)e(cid:272)ause dna is tra(cid:374)s(cid:272)ri(cid:271)ed fro(cid:373) (cid:1009)" to (cid:1007)"; so (cid:374)eeds to (cid:271)e at the (cid:271)egi(cid:374)(cid:374)i(cid:374)g constitutive promoter active in all cell types cell specific promoter active in specific cell types. If doing gene expression in a specific tissue choose this one enhancer dna sequence that increases the rate of transcription independent of its location and direction. In biotechnology, we make proteins that are beneficial to us; why we need to know about transcription.