BIOC 2580 Lecture Notes - Lecture 15: Isocitrate Dehydrogenase, Pyruvate Dehydrogenase Complex, Succinyl-Coa

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Lecture 15 tca cycle ~ stage 2 of oxidation. Acetyl coa = major end product of catabolism of fas, sugars, and certain aas. Tca effects the complete oxidation of acetyl coa co2 (second stage of respiration) Generates for each turn of cycle = 3 nadh, 1 fadh2 and 1 gtp (atp) Step 1: condensation of acetyl coa with oxaloacetate to form citrate (citrate synthase) Step 3: oxidative decarboxylation of isocitrate (isocitrate dehydrogenase) Isocitrate dehydrogenase reaction involves oxidation of alcohol ketone. Citrate = a tertiary alcohol, is a poor substrate for oxidation. Elimination of h2o from citrate gives a cis c=c bond. Hydration of cis-aconitate gives the secondary alcohol isocitrate. *the only tca step that forms a c-c bond. *methyl of acetyl-coa acts as the nucleophile once deprotonated by the enzyme. Via transfer of hydride from c-h of alcohol to nad+ followed by loss of coo gp as co2. Converts -ketoglutarate to high energy thioester succinyl coa.

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