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Lecture 19

BIOL 1090 Lecture Notes - Lecture 19: Phagocytosis, Cell Membrane, Triskelion

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BIOL 1090
Andrew Bendall

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How might certain proteins be removed from the circulation? How might some toxins be sequestered?
Central question:
Macromolecule transport within, between, and out of cells
Addition & removal of macromolecules
Multicellular organism!!
Made from single-celled organisms coming together
Shows how vesicles can have vesicles inside!
Big colony! (but have little colonies inside!0
Similar to how vesicles behave!
"Splat" when reach target and gets incoporated into it!
Burst paint balls
Types of "Vesicles"
If KDEL protein escapes --> COPI coat = retrograde transport back to ER
Retrieval of resident ER proteins
Rads ==> tethering
V-snare (vesicle) & T-snare (target) bind together to dock!
After docking --> vesicle fuses with target!
SNAREs ==> docking
Vesicular Transport
Transport proteins from TGN --> other vesicles (after they've gone through Golgi!)
Composed of 3 heavy & 3 light chains
Polyhedral lattice ==> basket-like structure that surrounds vesicle
Triskelions interact!
Selectively sorts the cargo!
CCV adaptor proteins located between lattice & cytosolic face of vesicle
Clathrin coat starts coming in from outside to form vesicle
Round up & pinch off --> exocytosis!
Clathrin forms "triskelion"!
Receptor-mediated exocytosis!!
Clathrin-coated vesicles (CCV)
Structure of clathrin (triskelions) makes curve automatically!
Dynamin loops around tubule (tubulase) and pinches vesicle off (pinchase)!!
GTPases --> ex: Dynamin!
Formation of endocytic clathrin-coated vesicles
Still same orientation tho! (b/c still facing outwards from cytoplasm!)
Also --> when vesicle pinches...C-terminal will be inside lumen again! O:
And then when it's thrown outside --> C-terminal faces OUT of the cytoplasm!
Ex: if C-terminal was in the lumen of the ER --> will always be inside the lumen!! (of vesicles,
Golgi, etc)
Orientation of a protein with respect to the cytoplasm & the interior of the compartments is
maintained during/throughout its travels through the endomembrane system!
Digestive enzymes
25~1000 nm
From H+-ATPase! (pumps H+ inside!)
4~4.6 pH inside
Hydrolytic enzymes - use acid hydrolases
Protective lining next to lumen! (protect itself from acid)
Lysosomal membrane = made of glycosylated proteins
Lecture 19 Vesicles, Lysosomes, Vacuoles &
March 21, 2016
8:30 AM
BIOL 1090 Page 1
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