BIOL 2400 Lecture Notes - Lecture 8: Guanine, Transect, Hydrogen Bond

33 views9 pages

rosemouse151

25 Dec 2014

School

Department

Course

Professor

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Dna may code for amino acids/protein (1. 5% of genome) : any change to the genomic: mutation sequence. : a pairs with t, c pair with g. *insert transitions and transversions (weighed 2x heavily as transitions) slide. : simple tandem repeats of 10-30 copies of differences in size detected among alleles how many copies are there? estimate size variation gel samples. Neutral molecular marker: single nucleotide polymorphism (snp: synonymous or silent mutation in third codon of 2nd triplet, still stays as alanine therefore silent mutation and neutral molecular marker. 5. run pcr product on capillary filled with acrylamide gel to determine its size. Nuclear sequence showing snp heterozygotes have two lower peaks. ** not asked on a test, just know it"s easy to do. Microsatellite/strs are usually in noncoding nuclear dna sequences only low strand of each copy shown vvvvvvv. Gel electrophoresis to measure levels of genetic variation in populations used long time ago.

Related Questions

5). The table shows five different mutations of a small section of a gene.

Use the base-pairing rules to complete the second column. For each mutation, write the sequence for any mRNA codons that will be changed as a result of the mutation in bold. Write any codons that wonât be changed in regular font.

Using the genetic code chart from question #3, identify any stop codons in the mRNA molecules â write these codons in red

For each mutation, write any amino acids that will be changed in the last column in bold font. Use red XXXs to indicate any amino acids that will be missing as a result of stop codons and use use regular font indicate any amino acids that will not be changed.

DNA (Template Strand)	mRNA codon	Polypeptide
Original DNA = GCAAGTACCTGA	CGU UCA UGG ACU	arg â ser â trp â thr
Mutation = GCCAGTACCTGA (nucleotide change underlined)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GCACGTACCTGA (nucleotide change underlined)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GCAAGTACTTGA (nucleotide change underlined)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GAAGTACCTGA (first C deleted)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GCAAGTACTGA (second C deleted)	____ ____ ____ ____	___ ___ ___ ___

ambergnu488

You have reviewed the human genomic sequence and decided to study the function of an unknown protein-coding gene. In order to start your research, you must obtain the DNA of this gene. The gene is ~5kb and you are planning to clone the DNA in an expression vector to further analyze protein functions and interactions.

You identify a protein that is bound to your protein of interest through a specific protein sequence within it, and you notice that this sequence is mutated in several cases of cancerous liver cells, suggesting that the two proteins do not bind in cancer cells due to this mutation. In order to confirm that this sequence mutation is involved in altered protein interaction, what type of experiment should be conducted?

QUESTION 3

What is the purpose of chromatin-remodeling enzymes in human cells?

The enzymes control the rate of translation of an mRNA transcript

The enzymes add phosphate or methyl groups to histone tails

The enzymes slide nucleosomes farther apart or closer to each other

The enzymes send damaged proteins to the proteasome for degradation

The enzymes cause DNA looping to occur

QUESTION 10

If you can drink milk as an adult, it means that you have inherited a mutation in the an enhancer for your lactase gene (the gene that encodes the enzyme you need to break down lactose). Predict the effect of this mutation:

The mutation changes the number of domains in the enzyme, which makes it work more efficiently

The mutation changes the amino acid sequence of the lactase protein

The mutation increases the number of copies of the lactase gene that will be found in your genome

The mutation changes whether the lactase sequence is found in an intron or exon

The mutation affects the expression of the lactase gene

QUESTION 12

What determines where in the genome a transcription regulator will bind?

Transcription regulators bind to the 5' UTR region of a gene

Regulators bind via complementary base-pairing to certain DNA molecules

Covalent bonds form between the transcription regulator and the atoms of the DNA backbone

Every eukaryotic gene has a different transcription regulator that will bind to the 5' end of the gene

Transcription regulators bind to specific DNA sequences via multiple weak non-covalent interactions

QUESTION 15

What is the histone code used for?

		Phosphorylation and acetylation of DNA affect its ability to be compacted
		Changes to the sequence of DNA change whether DNA will wrap around histone proteins
		Covalent modifications of histones affect the ability of the transcription initiation complex to form
		Histones provide the codon sequences needed for translation to occur
		The histone code affects which amino acids will get added to a polypeptide

BIOL 2400 Lecture Notes - Lecture 8: Guanine, Transect, Hydrogen Bond

Document Summary

Get access

Related Documents

BIOL 2400 Lecture Notes - Lecture 5: Gene Duplication, Chromosomal Inversion, Rna Splicing

BIOL 2400 Lecture Notes - Lecture 1: Environmental Disease, Mutation Rate, Birth Weight

BIOL 2400 Lecture Notes - Lecture 3: Dna Paternity Testing, Genbank, Gang Of Youths

Related Questions