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21 Apr 2019
You have reviewed the human genomic sequence and decided to study the function of an unknown protein-coding gene. In order to start your research, you must obtain the DNA of this gene. The gene is ~5kb and you are planning to clone the DNA in an expression vector to further analyze protein functions and interactions.
You identify a protein that is bound to your protein of interest through a specific protein sequence within it, and you notice that this sequence is mutated in several cases of cancerous liver cells, suggesting that the two proteins do not bind in cancer cells due to this mutation. In order to confirm that this sequence mutation is involved in altered protein interaction, what type of experiment should be conducted?
You have reviewed the human genomic sequence and decided to study the function of an unknown protein-coding gene. In order to start your research, you must obtain the DNA of this gene. The gene is ~5kb and you are planning to clone the DNA in an expression vector to further analyze protein functions and interactions.
You identify a protein that is bound to your protein of interest through a specific protein sequence within it, and you notice that this sequence is mutated in several cases of cancerous liver cells, suggesting that the two proteins do not bind in cancer cells due to this mutation. In order to confirm that this sequence mutation is involved in altered protein interaction, what type of experiment should be conducted?
Beverley SmithLv2
23 Apr 2019
