BIOM 4180 Lecture Notes - Lecture 4: Transfection, Phospholamban, Guide Rna

• School: University of Guelph
• Department: Biomedical Sciences
• Course: BIOM 4180
• Professor: Glen Pyle

Document Summary

3 major ca++ transporters involved in moving ca++ out of the cytoplasm. [1] serca (sarco/endoplasmic reticulum ca atpase: move ca++ back into sr. [2] na/ca exchanger (ncx: move ca++ one way, and na+ the other. In the relaxation phase, ca++ is moved out of the cell, while na+ is moved into the cell: the directionalities can reverse. In most animals, the serca does the most work to move ca++ (70-90%) The na/ca exchanger does a smaller portion of the work (10-30%) The sl caatpase does a very small fraction (0-4%) [3] ca++ uniporter found across mitochondria membrane (sl ca++ atpase: ca++ not only initiates muscle contraction, but also signals the mitochondria for more atp production, sustained high ca++ allows mitochondrial uptake of ca++ 2 ca++ bind to the cytoplasmic side of the transporter: causes a conformational change to expose the atp binding site, atp binds to the exposed pocket. The atp is dephosphorylated to adp and alters the transporter structure.