BIOL 2500 Lecture Notes - Lecture 19: Recombinant Dna, Exact Sequence, Cloning
28 May 2016
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Document Summary
Speci c dna sequences are recognized using recombinant dna technology. To discover the molecular basis for mutant phenotypes, researchers must be able to clone the genes they have identi ed. Recombinant dna technology provides the tools for proceeding from a mutant phenotype to. Recombinant dna technology is a set of techniques for amplifying, maintaining, and manipulating dna sequences in vitro and in vivo. The major challenge of recombinant dna technology is to. It allows researchers to divide the genome into smaller segments to be analyzed. Reassembled to provide a molecular view of genes and genomes. 6: assay the phenotypic effects of the introduced dna. Each restriction enzyme recognized a speci c dna sequence at which it cuts both strands of the sugar-phosphate backbone of dna. They were rst identi ed in bacterial cells, where they function as protection again coral infection. Restriction-modi cation systems methylate the restriction enzyme recognition sequences in bacteria, to protect the bacterial sequences from digestion.
