CHEM 1300 Lecture Notes - Lecture 4: Chris Paul, Spinach, Molar Mass

• School: University of Manitoba
• Department: Chemistry
• Course: CHEM 1300
• Professor: Sarrah Vakili

Document Summary

Date of experiment: 7th of may 2017 (wednesday 1:30pm) Using the spectrophotometer to identify compounds present in spinach and determine the iron content of spinach. Data obtained by using the spectrophotometer on the spectrum of the spinach extract is shown on the graph below. 0 a b s o r b a n c e. Table 1: final concentration of fe (iii) with the corresponding absorption on wavelength. 1 ml fecl3 : 4 ml hcl : 2 ml fecl3 : 3 ml hcl : 3 ml fecl3 : 2 ml hcl : 4 ml fecl3 : 1 ml hcl : 5 ml fecl3 : 0 ml hcl : Calculations of concentration of fe (iii) in test tube 1. Calculations of [fe3+] using data from test tube 1: Initial volume of fecl3 = 1 ml = 0. 001 l. Final volume of fecl3 = 10 ml = 0. 01 l. [(0. 00025m) x (0. 001l)] = [ cf x (0. 01l)]