FABS 210 Lecture Notes - Lecture 9: Lac Operon, Operon, Palindrome
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16 Jan 2018
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Template dna - sequence you wish to copy. It will make a mistake every 10000 base paris. Taq polymerase-the optimal function is 72. it is a puri ed enzyme. If deals with the negative charges on the phosphates, you also need mg for the enzyme to actually function. Taq polymerase: does not lose this structure even at 95. Proteins begin to denature, the proteins can start to unfold in your cells ex- if you have a fever. At 42 e. coli becomes heat shocked". You can kill bacteria by heat shocking. Allows primer to bind to the template. 1 kbp per minute when incubating it at 72 degrees. Cool to 60 and allow the primers to bind. Heat back up to 72 degrees and the cells elongate. Stringency-the closer you get to the melting point of the primers the more speci c it will be. Ampli cation: doubling of the amplicon after each round.
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