BIOL110 Lecture Notes - Lecture 5: Erwinia, Food Microbiology, Microbial Ecology

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purplegerbil552

14 Sep 2017

School

University of Waterloo

Department

Biology

Course

BIOL110

Professor

Marcel Pinheiro

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Document Summary

Growing different bacterial cultures in different agar mediums. Much of the study of microorganisms depends on their ability to grow in different types of culture mediums. A culture medium is a liquid or a solid preparation used for the growth of bacterial cultures. A culture is prepared with selective ingredients that help with the growth and inhibition of certain types of bacteria. There are several types of media can be prepared in order to monitor the growth of bacterial colonies. Selective, differential and enriched media are three mediums that were used in our lab to grow bacterial colonies of different species. After one week the abundance and the morphology of the bacterial cultures was recorded. There were three different types of growth mediums used in this lab. Selective media: selective media allows the growth of certain types of organisms while inhibiting the growth of other organisms.

Related Questions

Question 1

A microorganism's ability to use a harmful pesticide as a source of carbon and energy is an example of

Select one:

a. chemoautotrophy

b. nitrogen fixation

c. bioremediation

d. photoautotrophy

e. biosynthesis

Question 2

Autotrophs and heterotrophs differ in

Select one:

a. their ability to utilize CO2 as a sole carbon source.

b. their ability to incorporate CO2 into cells.

c. only a few enzymes.

d. their ability to synthesize precursor molecules.

e. their ability to utilize CO2 as a sole energy source.

Question 3

If the amino acid valine constituted approximately 4% of the total cell protein of a bacterium, how much valine should be added to a defined medium in order to grow 1 gram of this organism?

Select one:

a. 0.2 mg

b. 2 mg

c. 20 mg

d. 40 mg

e. 200 mg

Question 4

Transport of HPO₄ along with a proton (H⁺) is an example of

Select one:

a. antiport

b. uniport

c. facilitated diffusion

d. symport

e. group translocation

Question 5

The energy required for transport via ABC transporters is derived from

Select one:

a. a proton gradient

b. an electrical charge separation across the membrane

c. ATP hydrolysis

d. a symport mechanism

e. a high energy phosphate bond of PEP

Question 6

The spread plate method would clearly be preferred over the pour plate method when isolating colonies of

Select one:

a. anaerobic bacteria

b. thermophilic bacteria

c. bacteria from temperate lake zones

d. purple non-sulfur bacteria

e. agar-degrading bacteria

Question 7

Enrichment culture procedures for many microorganisms require intial growth in liquid media, followed by growth on solid media. The solid media is used to

Select one:

a. provide proper growth conditions.

b. isolate single colonies of the microbes.

c. get rid of competing microorganisms.

d. isolate strict aerobes.

e. enrich for specific nutrients.

Question 8

The optical density of light scattered by bacteria is a very useful tool for estimating population size. What can alter the bacteria's light-scattering properties?

Select one:

a. Cell volume depending on its growth stage

b. The condition of being absent of microorganisms.

c. The amount of light projected from the room.

d. The standard curve

e. Standard deviation from growth curve.

Question 9

What is true about a bacterial colony?

Select one:

a. It arises from an individual cell and represents an isolate strain.

b. Colonies, and CFU (colony forming units) on streak plates represent a diverse microbial culture.

c. In order to obtain a colony, on must perform cloning steps.

d. Axenic cultures to not represent CFUs or colonies.

e. Colonies require differential media in order to separate them from the population.

Question 10

What is a biofilm?

Select one:

a. All cells in a population achieve a steady state

b. A specialized, surface-attached, collaborative community of bacteria

c. A complex media made of the chemical building blocks that a cell needs

d. A type of anaerobic growth chamber.

e. A type of spore

sapphireguinea-pig180

The epiglottis acts as a

voice box.

filter for microorganisms and debris.

cover over the larynx.

producer of mucus.

ANSWER:

(Level 1, Objective 1)

Collectively, the nose, oral cavity, pharynx, epiglottis, and larynx make up the

nasopharynx.

oropharynx.

laryngopharynx.

upper respiratory system.

ANSWER:

(Level 1, Objective 1)

The cilia present on cells of the lower respiratory system function by:

trapping microorganisms and foreign debris.

propelling microorganisms and particles out.

preventing colonization by potential pathogens.

all of the above.

ANSWER:

(Level 1, Objective 2)

The most common causative agent of a swollen and painful oropharynx is

Streptococcus pyogenes.

Arcanobacterium haemolyticum.

Corynebacterium diphtheriae.

Rhinovirus.

ANSWER:

(Level 1, Objective 3)

Infections associated with cystic fibrosis are due to inflammation of the:

bronchi.

bronchioles.

lungs.

alveoli.

ANSWER:

(Level 1, Objective 3)

A physician sends a nasal washing for viral culture. This specimen is

unacceptable because it is contaminated with upper respiratory flora.

acceptable as long as it contains epithelial cells.

unacceptable if it is for the detection of influenza or respiratory syncytial viruses.

acceptable if it has a thick consistency and is yellow or green in color.

ANSWER:

(Level 2, Objective 4)

A patient is unable to produce adequate sputum for routine, acid-fast, and fungal cultures. The physician may attempt to obtain a/an

induced sputum.

transtracheal aspirate.

bronchoalveolar lavage.

pleural fluid.

ANSWER:

(Level 1, Objective 4)

9. Two throat swabs are received from a 6-year-old female with orders for a rapid screen for group A Streptococcus. One swab is tested using the group A Streptococcus rapid screen, and it is negative. The second swab is used to inoculate

a. sheep blood agar.

b. chocolate agar.

c. no culture is necessary.

d. a and b.

ANSWER:

(Level 1, Objective 6)

Which of the following disease states requires the addition of selective and/or differential medium to ensure recovery of potential pathogens from the respiratory system?

Pertussis

Cystic fibrosis

Laryngitis

a and b

all of the above

ANSWER:

(Level 2, Objective 6)

11. A throat culture provides the following results:

Moderate growth of alpha-hemolytic, catalase-negative, optochin-susceptible, gram-positive diplococci

Heavy growth of alpha-hemolytic, catalase-negative, optochin-resistant, gram-positive cocci

Heavy growth of gamma-hemolytic, oxidase-positive, glucose- and maltoseÂ-positive, gramÂ diplococci

You would report

moderate growth of Streptococcus pneumoniae and heavy growth of normal upper respiratory flora.

heavy growth of Streptococcus pneumoniae and heavy growth of normal upper respiratory flora.

heavy growth of Neisseria meningitidis and heavy growth of normal upper respiratory flora.

heavy growth of normal upper respiratory flora.

ANSWER:

(Level 3, Objective 7)

Yeast colonies in a sputum culture are considered significant when they

are present.

comprise 25% of the organisms present.

comprise 50% of the organisms present.

comprise 75% of the organisms present.

ANSWER:

(Level 1, Objective 7)

13. An 8-year-old male presents with a red and sore throat. The rapid group A Streptococcus screen is negative, and a sheep blood agar is inoculated. At 24 hours, there are no beta-hemolytic colonies present and moderate growth of normal upper respiratory flora. At 48 hours, the same culture results are present. Which of the following statements does not explain these results?

a. His sore throat is due to Haemophilus influenzae, and it will not grow on blood agar.

b. The culture was taken very early in the infection.

c. The patient received treatment prior to specimen collection.

d. The causative agent is not bacterial.

ANSWER:

(Level 2, Objective 8)

17. A quantitative bronchial alveolar lavage culture requires:

1. sheep blood agar.

2. chocolate agar.

3. 0.01 mL pipette.

4. 0.001 mL pipette.

a. 1 and 3

b. 1, 3, and 4

c. 2, 3, and 4

d. 2 and 4

ANSWER:

(Level 1, Objective 9)

18. A bronchial washing is plated to a BCYE. This agar should be examined under a laminar flow hood due to the potential for recovery of Legionella pneumophila and

a. Bordetella pertussis.

Francisella tularensis.

mould.

Pneumocystis jiroveci.

ANSWER:

(Level 2, Objective 9)

20. A physician submits a sputum with a culture request for Legionella. Select the growth medium that should be used to cultivate this organism.

a. 7H10

b. TCBS

c. BCSA

d. BCYE

ANSWER:

(Level 1, Objective 6)

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BIOL110 Lecture Notes - Lecture 5: Erwinia, Food Microbiology, Microbial Ecology

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