BIOL240 Lecture Notes - Lecture 5: Titer, Lethal Dose, Ribonuclease

• School: University of Waterloo
• Department: Biology
• Course: BIOL240
• Professor: Josh Neufeld

Document Summary

Plagues humans for a long time before they were even known. Bega(cid:374) i(cid:374) (cid:1005)8(cid:1004)(cid:1004)(cid:859)s (cid:894)to(cid:271)a(cid:272)(cid:272)o i(cid:374)fe(cid:272)tio(cid:374) (cid:373)osai(cid:272) (cid:448)irus(cid:895) Felix d"herelle discovered bacteriophages, walter reed and colleages shows yellow fever virus via mosquitoes. Intracellular obligate parasites, size 10 100 nm, genome 200000 nulceotides long. Exception small viruses (to amoebas): megavirus chilensis [greater than 1. 2 pairs, 1200 proteins), mimivirus [400nm, 1. 2 pair genome, 979 proteins], and pandoravirus [2. 5 pairs, 1 micron in diameter] Capsid around genome (capsomere proteins, genome + capsid = nucleocapsid) envelope. Capsids: helical or icosahedral or irregular in shape. Viral envelopes: enveloped virus [membrane covering nucleocapsid]. Replication: adhere stick to host cell, enter into host, uncoat and release genome, assembly create 6. Exit new particles leave cell: synthesis express and replicate genome new virus particles. Entering the cell (most important part in cycle) Hiv and cd4 receptors on t cells) Depe(cid:374)d (cid:448)ar(cid:455) o(cid:374) host (cid:272)ell [ e. g a(cid:374)i(cid:373)al (cid:448)iruses do(cid:374)(cid:859)t ha(cid:448)e to (cid:272)o(cid:374)te(cid:374)t (cid:449)ith a (cid:272)ell (cid:449)all]