BIOL240 Lecture Notes - Lecture 20: Exponential Growth, Serial Dilution, Chemostat
15 Mar 2019
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Document Summary
A spectrophotometer can also be used to determine the four basic phases of microbe"s growth curve in a culture over time. At higher turbidities, its more accurate to use viable cell density pour plate method using serial dilution. Lag phase may also be because of bacteria responding to new carbon sources. By analyzing the data from a growth curve, one can determine: generation time: the time to double the population in the exponential phase. If generation time is constant it is in the exponential phase. You first pick a value for on your y axis. This number must be in the linear region of expo phase. Next double it and make sure that this point on the y axis is also on the linear section. Find respective points on x axis and difference between these two are generation time in od vs viable cell density via plating, the stationary phase of viable will be high than od.
