BIOL240 Lecture Notes - Lecture 28: Protein Kinase, Ti Plasmid, Hpk
Document Summary
Atp dependent: response regulator will be dephosphorylated after some time by phosphatases or the rr does it itself. Different two component regulatory systems can allow microbes to respond differently to environmental stimuli. Pairing particular hpks and rrs, cells can better control which genes are expressed in response to cues from the environment. Isolated using a capillary tube filled with nutrients: microbes with normal chemotaxis will move into the tube. Those with mutations in chemotactic proteins will remain outside the tube (cap. Tube filled with semisolid agar and contains an attractant) Regulations of chemotaxis: response to the signal, control of flagella rotation, adaptation like for example when you are far away from a source, you must be very sensitive to changes of attractant conc. But as you move closer you must have a lower sensitivity to make out between conc. Is better: detecting signal intrinsic protein with complex on cytoplasmic side. Found in large numbers all over the cell.