BIOL240 Lecture Notes - Lecture 1: Tl;Dr, Committee For Accuracy In Middle East Reporting In America, Pyrophosphate
Document Summary
Genomics the discipline involving mapping, sequencing, analyzing and comparing genomes. Functional genomic - assigning function to unknown genes by analyzing biochemical and physiological effects of mutants. Genomics has been spurred by the development of recombinant dna protocols. Formats for storage of very large data sets. Sanger/dideoxy sequencing takes advantage of primer-specific dna synthesis activity of dna polymerase. First isolate the plasmid containing the cloned dna and denature it to provide a single-stranded template. Add a radiolabeled short oligonucleotide primer to this template, dna polymerase, and the four deoxyribonucleotide triphosphates ( dntp where n are the bases g, c, t, and a) The primer is complementary to vector sequence flanking one end of the cloned dna, anneals to the single-stranded template, providing a base-paired free 3" oh. Dna polymerase requires a free 3" oh to continue synthesis. Placing dideoxynucleotides into the dna synthesis mixture terminates the process with a labeled end-point nucleotide.
