BIOL308 Lecture 6: Lecture 6- DNA replication
11 Jun 2017
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Document Summary
Dna (rna) protein interactions very important. Dna: restriction enzymes, topoisomerases, telomerases, methylases, polymerases etc. Regulatory: binding to nucleic acids helicases (unwinding parental dna duplex during dna replication), histones etc. In both transcription (dna binding) and translation (rna binding) Dna and rna are negatively charged - binding proteins tend to be positively charged (e. g. histone) Dna and rna are surrounded by positively charged ions. When protein binds to nucleic acid, positively charged ions are displaced (energy requirement etc. ) Amino acids (protein) interacting with nitrogenous bases (dna): involves major and minor grooves. Describe the cis-acting elements and trans-acting factors involved in the process of replication initiation. Describe how ori sequences are located and studied. Efficient starts at a defined sequence of base pairs. Replication origin: recognized dna sequence necessary and sufficient for the beginning of replication bacterial and plasmid genomes often have only one more complicated in eukaryotic cell multiple replication origins. Each region served by one dna origin is a replicon.
