BIOL308 Lecture 3: Lecture 3
11 Jun 2017
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Describe how base pairs can be identified in the major groove based on h-bond donors and acceptors. A, b, and z dna structures and when each structure occurs in vivo. Minor groove shallow and broad (superficial) moderate depth, narrow. Low humidity (75%0 high salt high humidity (95%), low saly high mgcl2 (>3m), nacl or ethanol. Very shallow, virtually nonexistent sometimes called a single groove . In the presence of methylated cytosine; high humidity and low salt. Formed when purines make up one strand and pyrimidines the other, then a third strand can be accommodated (allows for this 3rd) Occurs in vitro, but also likely in vivo during dna recombination or repair. Being tested to block expression of genes in vivo looking at it wrt treatments for other diseases as well. Action of agents that denature dna vs. forces that keep dna strands together. What factors affect the tm of a dna fragment.
