BIOL308 Lecture 10: Lecture 10- DNA recombination
11 Jun 2017
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Document Summary
Transposition: mobile dna element moves from one site to another (donor and target site), usually little sequence similarity involved. Jumping dna or fragments or genes: transposition. Nonhomologous recombination: transposable (mobile, transposon) elements insert into dna that has no sequence homology with the transposon. However, there are preferred sequences (hot spots) that are targeted. Autonomous encode all the enzymes necessary to move, Transposon element insertions represent a major source of spontaneous mutations in drosophila melanogaster. Ultrabithorax mutant double thorax/wing mutation is due to insertion of a doc transposable element into the first exon of the ubx gene. Can find a lot of mutations due to transposon, in this case the element positioned into ubx gene and unique phenotype. Haemophilia a: insertions of l1 element (retrotransposon, which has homology with a retrovirus) into exon 14 of the factor viii gene in two of 240 unrelated patients with haemophilia a (kazazian et al.
