BIOL308 Lecture Notes - Lecture 4: Dna Polymerase Iii Holoenzyme, Klenow Fragment, Dna Polymerase I
Document Summary
List proteins (enzymes and other factors) involved in the process of. Explain the role of each of these proteins in replication. initiates replication at oric: dnaa, recognizes 4 dnaa boxes in oric, 10-20 dnaa proteins and 4 dnaa boxes form an initial complex. Initiates replication only if dna is negatively supercoiled easy to melt (stored energy: binding and opening occur at the 13 bp sequences and require additional. Replication is semiconservative: each daughter duplex will consist of one old and one new strand. Replication is bidirectional from the origin: 2 replication forks replication bubble leading and lagging strands. Subunit functions as a clamp; it associates with core at 3" end of the growing strand, increases the processivity of polymerase. Decatenation is the separation of catenanes (nearly completed daughter sequences equivalent to sister chromosomes in euk) decatenation is basically untangling of the two dna molecules.