CHEM267L Lecture Notes - Lecture 34: Histidine, Ninhydrin, Chief Operating Officer
5 Jan 2016
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During our lab, we were given unknown amino acid #6. This mixture contained two unknown amino acids, through which they were put through two tests: ion exchange chromatography (iex) and electrophoresis. Through these tests, the unknown identities were found to be glycine and aspartate. It is believe that these amino acids are the unknown due to the evidence found in the lab. Iex, we were required to compare the absorbance spectrums. Comparing where the spectrums on our absorption graph are to the electrophoresis, it is clear to see where each amino acid should go and where the ph changes to alkaline. Due to aspartate being acidic and its net charge being negative, it would elute near the acidic phase of the graph, which is verified by the plot. Glycine being neutral and having a relatively basic ph, it would elute near the alkaline portion of graph, which is verified by the plot.
