CHEM267L Lecture Notes - Lecture 13: Rhabdomyolysis, Psychrophile, Absorbance
5 Jan 2016
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Questions: if you were to disturb the resin bed right after adding the sample, the column will become disturbed and the bands would be undefined. Once these fractions are put into the chromatogram, your values will not create a peak definite to the proteins you are trying to graph. Also, if the resin is disturbed, the proteins could come into contact with eachother and combine. If this were to happen, the chromatogram is going to look very strange with no defined peaks. Bed volume is equal to ( r 2 xh . That means that any protein larger than 60000 daltons, will exclude with the void volume. Therefore due to catalase being a large protein of 220000 daltons, it cannot fit between the pores. Therefore it will exclude with the void volume. It looks as if catalase eludes at fraction 6. That means that the void volume must be in between fractions 1 to 6.
