BIOL 112 Lecture 5: BIOL 112 – Lecture 5
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BIOL 112 Full Course Notes
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Secondary/tertiary non-covalent bonds (hydrogen-bond), base stacking (non polar) stabilize. Flat, planar, largely non-polar bases stack on top of each other, excluding water. Ans: because all watson-crick purine-pyrimidine base pairs have the same geometries. (appropriate spacing, and geometry) The same regular distance between the strands. Regular flat stacking interactions between bases above and below. Goal: to get lots of copies of a specific dna molecule. (forensics, genetic test) 4 ingredients: dna template, primers short dna, flag region of interest to be amplified, taq dna polymerase enzyme, dntps - nucleotides amplify, water. 3 steps: denaturation add 95 c heat. 2 strands separate; break hydrogen bonds: annealing 60 c, primers bind. Cool enough so short stretch to bind to template: elongation/extension 72 c. Dna primers bind to dna strands by matching base pairs, and antiparallel orientation. Provides the start of how dna can be replicated so we can replicate. Dna in vitro to make many copies of specific stretch of dna.
