BIOL 200 Lecture Notes - Lecture 2: Bright-Field Microscopy, Dark Field Microscopy, Green Fluorescent Protein
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Definition of life: controversial every discipline may have a slightly varied definition, eve(cid:396)al (cid:272)ha(cid:396)a(cid:272)te(cid:396)isti(cid:272)s (cid:396)e(cid:395)ui(cid:396)ed (cid:271)y (cid:271)iologists fo(cid:396) (cid:862)life(cid:863) Take energy from the surroundings and transform it. Scale of things: micrometers (microns or 10-6 meters) Robert hooke (17th century) (cid:272)oi(cid:374)ed the te(cid:396)(cid:373) (cid:862)(cid:272)ells(cid:863) ~200 years later, schleiden and schwann used (cid:373)i(cid:272)(cid:396)os(cid:272)opes to esta(cid:271)lish (cid:862)(cid:272)ell theo(cid:396)y(cid:863) Resolution: defined as the closest spacing of two points which can be distinguished as separate entities. Note: the smaller you get, the more magnification and resolution you need to see an object. Major classes of microscopy: brightfield light microscopy, fluorescence light microscopy (emitted lm, transmission electron microscopy (tem, scanning electron microscopy (sem) Light microscopy: used to see live cells, color and whole tissues, resolution limit = 0. 2 m (usually) Types: bright field, dark field, phase contrast, differential interference-contrast (dic) Colored light (or lasers) are used to excite photons that are then emitted by sample and viewed.
