MDST*2020 Lecture Notes - Lecture 1: Sanger Sequencing, Genetic Linkage, Omics
Document Summary
Many exam and midterm questions will be from tutorial and quizzes. Gene maps, put on everything about these genes i. e. positions. Genetic map positions genes based on frequency of recombination need to now what genetic maps are very important. Dye fluorescence- detected by sequencing matching as fluorescent. These procedures do not include termination of chain elongation by ddntps. 454 only need to know big details (what"s on slide just said another way ) get dna break into small and workable pieces (1000 b. p. ). Then denature single stranded and mix them with beads . then encapsulate and you can capture single molecule od na- each bead will be in viislce then use pcr amplification. Summary 454 (not on slide: does not need ddntp, this tehcnologuy measures the release of ppi in the well, no harsh chemicals, allows for the sequencing of long templates, sutable for de novo sequencing of genomes. Makes clusters of unique dna by complicated technology.
