BCH 2333 Lecture 13: Michealis - Menten

• School: University of Ottawa
• Department: Biochemistry
• Course: BCH 2333
• Professor: Christopher Boddy

Document Summary

V = k[s] v = rate, k = rate constant. Order of reacion = sum of exponents of reacing species v=k[s] 1st order v=k[s][t] 2nd order v=k[s]3 3rd order. Linear phase rate increases as [s] increases. Saturaion phase [s] does not impact the rate. E + s e*s, like ligand binding. E+s p +e, like a chemical reacion. Rate of p formaion = dp/dt = k2*[es] From the steady state approx. , [e] = (k-1 + k2) [es]/k1 [s] o o. So, [et] = (k-1 + k2)[es]/k1 [s] +[es] Solve for [es] [es] = [et] / (1+ (k-1 + k2)/k1[s]) = et[s]/([s] + (k-1+k2/k1) Km = k-1+k2/k1 = michaelis constant o o o o o. Since dp/dt = k2[es] dp/dt = k2[et][s]/([s]+km) Vmax = kcat [et] dp/dt = vmax[s]/([s]+km) = mm equaion! Someimes called synthetase - tells you that they always use atp! Assigned an ec number which deines exactly which reacion it catalyzes o o.