BIO 1140 Lecture Notes - Lecture 9: Uncoupling Protein, Intermembrane Space, Thermogenesis

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Transfer of electrons from the two electron carriers nadh and fadh2 the. Electrons on nadh move into complex 1 by pumps. Then travels with the aid of the non polar ubiquinone from complex 1-2 restricted to core of mitochondrial membrane. From 3-4 the electrons are carried by cytochrome c. Electrons are transferred from complex 4 to oxygen producing water. No proton pumps for the electrons in complex 2. Less proton pumping for electrons carried by fadh2 than nadh. Complexes 1, 3 and 4 and ubiquinone can pump electrons. Protons do no operate in reverse i. e. if it is pumped across the membrane it stays across the membrane. If you block complex 4 of the etc you prevent the proton pumping and prevent atp synthesis like in cyanide. Moves protons across the membrane form the high concentration in the intermembrane space into the lower levels of the matrix.

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