BIO 2133 Lecture Notes - Lecture 7: Functional Genomics, Model Organism, Gametogenesis

• School: University of Ottawa
• Department: Biology
• Course: BIO 2133
• Professor: Colin Montpetit

Document Summary

Phenotypic screens are not supposed to be used to determine genetic ratios. Reverse genetics: putative gene -> induce mutations (or just functional genomics) -> screen genetic variates -> gene function (confirm sequence: hypothesize that you have found the correct gene that is involved with the phenotype. Forward genetics: start experiment with interest in a phenotype without knowing if there is a gene biological phenomenon (genetic variants?) > induce mutations (if you can mess up the genome, you can confirm the genome) -> screen genetic variants confirm transmission and map it -> dna sequence and gene function controlled experiment. Screen: each member of the population is examined (does it fit the phenotype criteria that have been set up?) We want to: control when mutations occur cause mutations during gametogenesis capture the gametes by fertilization identify all progeny with potential mutations identify mutants of interest. Rules of inheritance (cid:373)eiosis, ge(cid:374)ot(cid:455)pes (cid:894)so(cid:373)e (cid:272)a(cid:374) (cid:271)e e(cid:454)pressed to phe(cid:374)ot(cid:455)pes, so(cid:373)e do(cid:374)"t(cid:895)