BIO 2133 Lecture Notes - Lecture 3: Gene Mapping, Ascocarp, Genetic Linkage
4 Sep 2018
School
Department
Course
Professor
Document Summary
Laboratories 3 and 4 are both dedicated to the assessment of gene recombination and gene mapping in the fungus sordaria fimicola. Laboratory 3 will assess a simpler case that involves crosses between the wild type and either one of two mutants, and laboratory 4 will look at crosses between the two mutants. This stepwise approach from a simpler to a more complex system should facilitate your comprehension of the underlying principles behind the important and common techniques that are gene recombination and gene mapping. The present worksheet was largely inspired from glase, j. c. (1995) a study of gene linkage and mapping using tetrad analysis in the fungus sordaria fimicola. Hands-on experience: acquire experience with fungal life cycle stages and with sterile" methods for manipulating microorganisms, calculate the map distance between a gene and the centromere of the same chromosome. This laboratory may seem straightforward as it essentially involves bursting bags" (perithecia) containing ascospores so they can be counted.
