BIO 2133 Lecture Notes - Lecture 11: Structural Genomics, Genomic Library, Functional Genomics
Document Summary
Encompasses structural genomics, functional genomics, and comparative genomics. Structural genomics focuses on genomes and analyzing nucleotide sequences to identify genes and other important sequences such as regulatory elements. Identification of open reading frames requires translation of all 3 reading frames. By looking at the open reading frames, we can start translating and predicting the sequences into proteins and compare it to other proteins to identify function. For every 1 plasmid that you use, it will incorporate 1 dna fragment: transform the bacteria with the plasmid and grow it on a plate. When the bacteria grows, there will be many colonies. Each colony represents a part of your genome: take each bacteria clone / colony and sequence every fragment involved. When we first identified the location of the gene in the genome, we looked for dna markers that were linked to it.