BIO 2137 Lecture Notes - Lecture 3: Taq Polymerase, Agrobacterium Tumefaciens, Thermus Aquaticus
18 Oct 2016
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Document Summary
Rocks, on people, bottom of ocean, km above the earth i. e. e. coli (colon) leading cause of food borne illness. Great metabolic diversity, different ways to get energy. Lots of ribosomes but smaller than eukaryotic ones (70s vs 80s) (s is for size sometimes shape) Generally lack of membrane bound organelles or membrane compartments. Expect photosynthetic prokaryotes like cyanobacteria and purple bacteria. Has up to 90% peptidoglycan in wall, positive. Transformation - dna from the environment i. e. from dead cells. Transduction - dna transferred via viruses from one host to the next. Conjugation - plasmids often carry genes that confer a selective advantage i. e. antibiotic resistance. Genetic transfer can be even between domains, lots of mixing. Can survive for long periods in harsh conditions. Can be used as bioweapons to botox (botox is a poison) Obtain energy from light and carbon to form co2. Anaerobes lack an aerobic pathway and cannot use oxygen. Facultative - can grow in presence or absence.
