BIO 3124 Lecture 19: TOPIC 19 CLINICAL MICROBIOLOGY
24 Nov 2016
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Document Summary
Establish and confirm the etiology of the disease. Track the progression of the infection in the patient. Biochemical tests - make a biochemical fingerprint of organism. When antibodies react with multiple epitopes on soluble antigens, there is formation of networks which generate an insoluble precipitate. Precipitation reactions can take place in solution or in gels such as agar. The precipitate formation is influenced by the concentration of ag - ab. Cant form aggregates if no optimal ratio of antibody and antigen. Latex beads have a high valency + sample containing antigen = you would get agglutination, etc (positive result) Add a limiting concentration of complement proteins (c1-c9) Impt to add limiting amount of complement proteins. Will start classical cascade - so complement fixation. Complement proteins will not be used if no complex formed. Test that determines whether complement was used as a result of specific binding of ab to ag. Used to detect the presence of antibodies or antigens.
